Characterization of the involved receptors in immature and LPS-differentiated dendritic cells by using selective antagonists.

Cells were pre-incubated for 30 min with the A1 receptor antagonist DPCPX, the A2 receptor antagonist ZM241385, and the A3 antagonist MRS1220 at a concentration of 10−6 M. Ca2+ transients were analyzed, and the ratio after stimulation with AMP at a concentration of 10−4 M for 10 s is given. Data are means ± SE (n = 3). Actin polymerization after stimulation with AMP for 25 s was measured and calculated as the ratio between the medium control and stimulated cells. Data are means ± SE (n = 3). The chemotactic index after stimulation with AMP at 10−5 M was calculated as the ratio between stimulated cells and cells incubated with medium. Data are means ± SE (n = 3). TNF-α, IL-12p70, and IL-10 release after costimulation with LPS (3 µg/ml) and 10−4 M AMP was calculated, and cytokine content is given as pg/ml/0.2×106 cells. Data are means ± SEM (n = 3).
#p<0.05 compared to control,
*p<0.05 compared to AMP-stimulated cells.