Detection of Neoplasms by Metagenomic Sequencing of Cerebrospinal Fluid

Detection of Neoplasms by Metagenomic Sequencing of Cerebrospinal Fluid Wei Gu, MD, PhD^1,2*, Andreas M. Rauschecker, MD, PhD³, Elaine Hsu, BS¹, Kelsey C. Zorn, BS⁴, Yasemin Sucu, BS¹, Scot Federman, BS¹, Allan Gopez, BS¹, Shaun Arevalo, BS¹, Hannah A. Sample, BS⁴, Eric Talevich, PhD⁵, Eric D. Nguyen, MD, PhD¹, Marc Gottschall, BS¹, Bardia Nourbakhsh, MD, MAS⁶, Carl A. Gold, MD, MS⁷, Bruce A.C. Cree, MD, PhD, MAS⁸, Vanja Douglas, MD⁸, Megan B. Richie, MD⁸, Maulik P. Shah, MD, MHS⁸, S. Andrew Josephson, MD⁸, Jeffrey M. Gelfand, MD, MAS⁸, Steve Miller, MD, PhD¹, Linlin Wang, MD¹, Tarik Tihan, MD, PhD⁹, Joseph L. DeRisi, PhD^4,10, Charles Y. Chiu, MD, PhD^11,12, Michael R. Wilson, MD, MAS⁸* ¹Department of Laboratory Medicine, University of California San Francisco, CA 94107, USA ²Department of Pathology, Stanford University, CA 94305, USA ³Department of Radiology and Biomedical Imaging, University of California San Francisco, CA 94107, USA ⁴Department of Biochemistry and Biophysics, University of California San Francisco, CA 94107, USA ⁵DNANexus, Mountain View, CA 94040, USA ⁶Department of Neurology, Johns Hopkins University, MD 21287, USA ⁷Department of Neurology, Stanford University, CA 94305, USA ⁸Weill Institute for Neurosciences, Department of Neurology, University of California San Francisco, CA 94107, USA ⁹Department of Pathology, University of California San Francisco, CA 94107, USA ¹⁰Chan Zuckerberg Biohub, San Francisco, CA 94107, USA ¹¹UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, CA 91407, USA ¹²Department of Medicine, Division of Infectious Diseases, University of California San Francisco, CA 94107, USA * Corresponding authors: Michael Wilson, MD, MAS UCSF Department of Neurology 675 Nelson Rising Lane, NS212 San Francisco, CA 94158 [e-mail] Wei Gu, MD, PhD Stanford University, Department of Pathology 3373 Hillview Ave, 218 Palo Alto, CA 94304 650-723-1914 [e-mail]