RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in worms exposed to the nematicide cyclobutrifluram.

Plant-parasitic nematodes can cause a hugh damage and crop loss becoming a threat for food security. We use Caenorhabditis elegans as a model organism to study the effect of the new nematicide cyclobutrifluram. RNA for two different biological replicates was isolated from animals of each condition: nematicide exposure/ no exposure; and then sequenced to detect differentially expressed genes in exposed vs. non-exposed samples.