AMPKα2 binds to chromatin to regulate gene expression in ALL cells [ChIP-seq]

To identify genome-wide genes regulated by direct association of AMPKα2 to chromatin, ChIP-Seq assays were performed using HA (HA-AMPKα2) and RPB1 (RNA Pol II) antibodies in CCRF-CEM/HA-AMPKα2 (CN2) cells treated with glucose deprivation or no treatment controls. ChIP-seq analysis identified around 600 genes to which loci AMPKα2 was directly bound; many of these genes with HA-AMPKα2 recruitment overlapped with genes exhibiting RNA Pol II enrichment. ChIP-seq (using HA and RNA pol II antibody) in CCRF-CEM/HA-AMPKa2 cells treated with vehicle or Glucose deprivation condition.