Determination of gene expression levels.
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https://figshare.com/articles/dataset/_Determination_of_gene_expression_levels_/352166
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Relative expression of the csgD, csgB, adrA and bcsA genes determined by Real-Time PCR on RNA extracted from overnight cultures. 16S RNA transcript was used as reference gene. ΔCt values between the genes of interest and 16S RNA were set at 100 for MG1655 in LB1/4 medium, and transcript levels in other strains and/or growth conditions are expressed as relative values. Experiments were repeated at least three times, each time in duplicate; standard deviations were always lower than 5%.
创建时间：
2012-02-16
