Plasma levels in 18-hour fasted mice receiving orally saline (CTRL), fructose (2 g/kg), leptin (3 ng/g) or fructose with leptin.

Mice were killed 4 hours after gavage, blood was collected, processed as described inMaterial and Methods and used to determine biochemical parameters. Data are expressed as mean±SEM of n = 8 mice per group. One-way ANOVA followed by a multiple comparisons Tukey t-test were used for statistical analysis. *P<0.05, **P<0.01 vs. CTRL; #P<0.05 vs. fructose alone.