Genes and transposons expression profiling of wild-type Bone Marrow Derived Macrophages (BMDMs) after VSV and HSV infection.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an emerging and highly pathogenic coronavirus that causes coronavirus disease (COVID-19), and might even lead to death. The long terminal repeat of the endogenous retrovirus (LTR ERVs), a type of mammalian genome elements derived from ancient viruses which infected the host and now accounts for 8% of human genome, are implicated in multi viral pathogenesis and host immune responses. However, their expression patterns and functions during SARS-CoV-2 infection still remain to be uncover. We first identified that SARS-CoV-2 infection could upregualted the expression of TEs and ERVs from the published RNA-seq data. Then in this study, we also conducted the genome-wide analysis of ERVs from wild-type mice Bone Marrow Derived Macrophages (BMDMs) after VSV and HSV infection. Collectively, these results provides the first glimpse into the expression patterns and potentail roles of ERVs after viral infection, and would serve as a valuable resource for future studies on SARS-CoV-2 infection and pathogenesis. Overall design: To investigate whether VSV and HSV infection could induce the expression of ERV and TEs, we separated the BMDMs from wt BALBC/6J mice and induced them with GM-CSF for one week. We then performed gene expression profiling analysis using data obtained from RNA-seq of WT BMDMs with VSV and HSV infection for 12 hours. Comparative gene expression profiling analysis of RNA-seq data for VSV, HSV and MOCK group.