Fluorescence properties of EGFP compared to mRuby, its predecessors and other monomeric fluorescent proteins emitting in the orange to red spectral region.

aProduct of QY and Emol compared to the brightness of EGFP (53,000 M?1 cm?1��0.6) [41].bConcentration of the red chromophore deduced from the protein concentration as determined by colorimetric methods.cConcentration of the red chromophore determined by the alkaline denaturation method [42].dConcentration of the red chromophore determined by the dynamic difference method[14].eValues from reference [14].