CBTi-seq profiles cell type and splicing patterns in adult human testicular cells.

Spermatogenesis is a highly complex and precisely regulated process. Moreover, alternative splicing (AS) is widely prevalent in the testis, but the regulations of AS in spermatogenesis is only little explored. As methodological validation, we performed random cell picking from two male donors with obstructive azoospermia (OA) and collected 192 adult testicular cells Overall design: we performed random cell picking from two male donors with obstructive azoospermia (OA) and collected 192 adult testicular cells, then multiplexed sequencing by CBTi-seq