Next generation sequencing facilitates quantitative analysis of pAd-KLF5- and pAd-Control-infected mouse VSMC transcriptomes

Purpous: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathway. The goals of this study are to compare different gene expression between pAd-KLF5- and pAd-Control-infected mouse VSMC. Methods: adenovirus vectors either encoding KLF5 or empty adenovirus vector were transfected into mouse VSMCs. shSirt6 transfected or shControl transfected VSMCs samples were performed RNA sequencing (RNA-seq) analysis, functional enrichment analyses of gene ontology (GO) and gene set enrichment analysis (GSEA). Results: eIF5a is found to be highly expressed and predictive target of KLF5 in VSMCs. KLF5-enhanced VSMCs demonstrated cell proliferation and were noted to have promoted cell cycle progression between the G2 and M phases. In accordance with the gene set enrichment analysis (GSEA) according to RNA-sequencing, ATP biosynthesis and cell redox homeostasis was upregulated upon KLF5 overexpression. Conclusion: These findings provide mechanistic insights into the oncogenic activity of KLF5 for the first time. RNA profiles of test and control samples were generated by deep sequencing, using Illumina HiSeq 4000.