Identification of trans regulators of ADAR and A-to-I RNA editing using RNA-seq. Identification of trans regulators of ADAR and A-to-I RNA editing using RNA-seq

A-to-I RNA editing levels differ across tissues and cell types, but regulators of the editing process are largely unknown. We performed RNA-seq on M17 and HeLa cells with overexpression of different candidate Adar regulators and GFP as a control to assay editing level differences between overexpression and control. Overall design: To assay the effects of overexpressing candidate Adar regulators on A-to-I RNA editing levels, we transiently overexpressed ILF2, IL3, and STRBP in H293T cells and H293T cells stably overexpressing Adar2. We extracted RNA and made RNA-seq libraries. We sequenced two biological replicates of GFP overexpression (OE) controls and two replicates of ILF2, ILF3, and STRBP overexpression.