Methionine biosynthetic pathway genes metH and metZ affect curdlan synthesis in Agrobacterium sp. CGMCC 11546

Purpose: The goal of this study is compare the effect of MetH and MetZ gene in curdlan synthesis in Agrobacterium sp. CGMCC 11546. methods: The transcriptional and metabolomics analysis the function of metH and metZ in Agrobacterium sp. CGMCC 11546. Results: The transcriptional and metabolomics showed that the decrease of curdlan production in the ?metH and ?metZ mutants may be caused by the insufficient supply of energy ATP conclusion: MetH and MetZ play an important role in curdlan synthesis in Agrobacterium sp. CGMCC 11546 Overall design: The total RNA of each sample from the wild type strain and the gene deletion mutants after 24 hours fermentation, samples from metH deletion mutant named metH-1, metH-2 and metH-3; three biological repeats. samples from metZ deletion mutant named metZ-1, metZ-2 and metZ-3; three biological repeats; samples from the wild type strain named WT-1, WT-2 and WT-3; three biological repeats