Contribution of long-leaved proteins to proteasome load.

1Calculated as % of [3H]Leu incorporated into proteins (i.e., TCA-precipitable) after 16-h labeling/16 h.2% Of [3H]Leu released into TCA-soluble fraction/h, relative to the total radioactivity incorporated in proteins after 16-h labeling.3Long-lived protein degradation (as % of total [3H]Leu used for labeling/h) = Long-lived protein degradation (as % of [3H]Leu incorporated/h, column 3)×[3H]Leu incorporation (%/h, column 2)/100%.4Proteasome load from long-lived protein (LLP) = % of total [3H]Leu used for labeling/h (column 4)×% inhibition by ZL3ek (column 5)/100%.5Proteasome load total = LLP load on proteasome+short-lived proteins load on proteasome (Table 2, column 6).6LLP contribution to total load = LLP load on proteasome/total load×100%.