Effect of nanosecond pulsed electric field (nsPEF) on gene expression in TM3 Leydig cells. Effect of nanosecond pulsed electric field (nsPEF) on gene expression in TM3 Leydig cells

The aim of our study was to analyze cell response to nanosecond pulsed electric field (nsPEF) at the gene expression level. TM3 Leydig cells were used as a model. Transcriptomics analysis was carried out immediately after exposure (0 h) and 4 or 24 h after treatment. Overall design: TM3 Leydig cell line was exposed to nanosecond pulsed electric field (nsPEF). The generated pulses had quasi-rectangular shape, 60 ns pulse width, and 5.5 kV amplitude, which corresponded to an electric field of 14 kV/cm. In total, 80 pulses were applied at the repetition rate of 26.4 pulses per minute. Gene expression in nsPEF-exposed or sham-exposed TM3 cells was measured immediately after (0 h), 4 and 24 h after exposure. Four biological replicates were analyzed for each time point and each condition.