Simultaneous determination of diosmin and hesperidin in combined pharmaceutical preparation by synchronous fluorescence spectrofluorimetric method

Diosmin (DSM) and hesperidin (HSP) binary mixture was analyzed simultaneously by developing a sensitive, rapid, and simple synchronous spectrofluorimetric method. In methanol at ∆λ of 100 nm, the relative synchronous fluorescence intensities (RSFI) of both medications were measured. It was shown that these intensities were influenced by distinct experimental factors. The optimization and detailed study of these parameters were conducted. For DSM and HSP, respectively, the plots of synchronous fluorescence intensity-concentration were found to be rectilinear across the ranges of 0.5-5.0 µg/mL and 0.2-3.0 µg/mL. For DSM and HSP, respectively, 0.107 µg/mL and 0.048 µg/mL were the detection limits and 0.323 and 0.144 µg/mL were the limits of quantification. The method described in this study was effectively employed to estimate the quantities of both drugs present in commercially available mixed tablets. The results produced using this method were then compared favorably to results obtained using a different method for comparison.

Methods
Description of Method used for collection/generation of data: 

A series of 10 mL volumetric flasks were filled with aliquots of the DSM and HSP standard solutions covering the working concentration range shown in Table 1. Then, methanol was added to dilute them to the mark. By scanning both monochromators with 10 nm excitation and emission windows and a constant wavelength difference (Δλ) of 100 nm, SF spectra of the solutions were captured. For DSM and HSP, the SF spectra's intensities were calculated to be at 280 nm and 390 nm, respectively. The experiment was run in parallel with a blank one. To obtain the calibration curves, plotting of the RSFI with the final drug concentrations (µg/mL) was done. An alternative was to derive the corresponding regression equations.