Analysis of the Putative Nucleoporin POM33 in the Filamentous Fungus Sordaria macrospora

The POM33 protein of Sordaria macrospora is a nucleoporin considered of being an ER-protein that may function in ER-shaping and organization. In this study, we performed TagRFP-T pulldown experiments in S. macrospora strains expressing either POM33-TagRFP-T or the free TagRFP-T as control to identify putative POM33 interaction partners. Since with the SmSTRIPAK-component SCI1, POM33 was found, we expected with this approach to vice versa pull down SCI1. The results of the LC/MS analysis revealed mainly proteins that are located at the ER-membrane where they have functions in fatty acid biosynthesis, sterol biosynthesis, lipid metabolism or ER-shaping and maintaining its organization. These results gave us an overall picture about putative interactions of POM33 to specify its localization and function in S. macrospora hyphae.