Systemic immune dysregulation in hypertensive disorders of pregnancy persists years after delivery

Background: Hypertensive disorders of pregnancy (HDP), including preeclampsia and gestational hypertension, are associated with an increased risk of cardiovascular disease (CVD) later in life. Mechanisms that link HDP to CVD, however, remain unclear.
Methods: We used a high-dimensional single-cell mass cytometry approach to profile the distribution and functional responses of maternal immune cells in three groups of HDP cases and normotensive controls: antepartum, postpartum, and midlife. We used multivariable sparse modeling to distinguish HDP cases from controls.
Results: We accurately distinguished HDP cases from controls at all three study timepoints, with area under the receiver operator characteristic (AUROC) values of 0.814 for the antepartum group, 0.757 for the postpartum group, and 0.692 for the midlife group. We identified a persistent immune dysregulation signal among cases at all three timepoints, characterized by increased B cell frequency and decreased pSTAT3 response upo..., , # Data from: Systemic immune dysregulation in hypertensive disorders of pregnancy persists years after delivery

Dataset DOI: [10.5061/dryad.7m0cfxq7w](10.5061/dryad.7m0cfxq7w)

## Description of the data and file structure

Goal:

Identify whether the immune system is dysgregulated in case with hypertensive disorders of pregnancy by analysing the distribution and function of the maternal immune system using mass cytometry across three groups: antepartum, postpartum, and midlife.  

Mass cytometry:

Whole blood samples were collected into sodium-heparinized vacutainers. Blood aliquots were stimulated for 15 minutes at 37°C with either LPS (1μg/mL), IL-18 (100ng/mL), a cocktail containing IL-2, IL-4, and IL-6 (100ng/mL each), or PBS (unstimulated condition). Samples were with Proteomic Stabilizer (SmartTube) for 10 minutes and stored at -80°C until further processing for mass cytometry. 

Cell frequencies are reported as a percentage of mononuclear cells, with the exception of granulocyt..., This study was approved by the Stanford University Institutional Review Board, and all participants provided written informed consent, including consent to sharing of de-identified data in public databases. All data is de-identified.
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