Dissecting the process of human neutrophil lineage determination by using alpha-lipoic acid inducing neutrophil deficiency model

Neutrophil deficiency or differentiation disorder might cause serious diseases, such as neutropenia and neutrophilia. To reveal the molecular mechanism of human neutrophil lineage commitment, single cell RNAseq and bulk cells RNAseq were performed to reveal the characteristics and essential genes of neutrophil lineage determination. Overall design: To explore the molecular mechanism of early commitment of neutrophil progenitors derived from GMPs, we performed single cell RNAseq for GMPs derived early progenitors, early neutrophil bias progenitors (CD34+CD15- NbP1, CD34mid CD15mid NbP2, CD34int/-CD15+NbP3) gated from CD115-CD73-CD1C-CD116- cells and CD34midCD115+MoPs. In addition, we also performed bulk cell RNAseq for ALA treated NbP1/NbP2, ELK1 and S-ELK1 overexpressed NbP1. The single cell RNAseq and bulk cell RNAseq were performed as we described in previous published paper (PMID:29434353, 31443434). 10X single cell RNAseq was performed for human bone marrow derived Lin- CD371+ progenitors.