How does the root architecture of wheat influence the microbial community and activity in soil? – β-Glucosidase gradient from the root center towards the surrounding soil.

The data set includes the enzyme gradient of β-glucosidase from the root center towards the surrounding soil at different sampling times, soil depths, and spring wheat genotypes. Enzyme data was collected using the soil zymography according to Spohn et al. (2013). The spring wheat genotypes used (Rambla et al., 2022) form different root architectures (UQR012 = shallow root system, UQR015 = deep root system). Plants were grown in columns under controlled environmental conditions in a climate chamber and sampled at four sampling dates (4, 5, 6, and 7 weeks after sowing). Zymography was performed on the surface of soil segments at two soil depths (4.5 cm and 31.5 cm). The soil used originated from the upper 30 cm of an agricultural Haplic Luvisol near Selhausen (Germany).

Literature:
Rambla, C., Van Der Meer, S., Voss-Fels, K.P., Makhoul, M., Obermeier, C., Snowdon, R., Ober, E.S., Watt, M., Alahmad, S., Hickey, L.T., 2022. A toolkit to rapidly modify root systems through single plant selection. Plant Methods 18:2, 1-13.
Spohn, M., Carminati, A., Kuzyakov, Y., 2013. Soil zymography—a novel in situ method for mapping distribution of enzyme activity in soil. Soil Biol Biochem 58:275–280.

Research domain: Soil Sciences

Research question: None