L. pneumophila putative effectors that were experimentally examined.

aIn bold, genes that were validated to encode for effectors; in italics, genes that we failed to clone or express; in plain text, genes that encode proteins that failed to translocate.bThe machine learning cutoff of the classification score used for determining the list of highly confident effectors in each learning phase. In brackets is the number of predicted effectors with equal or higher score than the cutoff value. In total 103 putative effectors had cutoff values similar to those experimentally tested (some of the putative effectors overlap among phases).cValidated effectors/successfully expressed genes/genes tested.