Transcriptome profiling of SW480 cells upon treatment with siRNA against DIS3L2 and DIS3L2+TUT4+TUT7. Role of DIS3L2 in colorectal cancer cells

Several studies have described the crucial role of DIS3L2 and uridylation in cytoplasmic RNA surveillance, promoting decay of miRNAs, mRNAs and ncRNAs, and it has been documented that DIS3L2 dysfunction disrupts transcriptome homeostasis. This study investigates the transcriptome-wide impact of decreasing the expression of DIS3L2 or DIS3L2 and TUTs 4/7 in a colorectal tumorigenic context by performing Illumina mRNA-seq profiling of colorectal cancer cell line SW480 treated with siRNAs against the transcripts encoding these proteins. As control, a siRNA targeting the exogenous transcript of firefly luciferase (siLUC) was used.