Prion promoter tetracycline-transactivator expression: coronal sections (case 388.12)

Bright-field microscopy images of serial coronal brain sections showing prion promoter (Prnp) promoter expression in a bigenic Prnp-tetracycline-transactivator (tTA) mouse brain (case 388.12, adult female), using a driver-reporter construct in which the Prnp-tTA promoter regulates the expression of the E. coli derived LacZ reporter gene encoding β-galactosidase, which is visualized histologically using X-gal (5-Bromo-4-chloro-3-indolyl β-d-galactopyranoside) as a substrate. Methodological details are provided in Boy et al., Neuroimage 33:449-62, 2006. Our data show that the Prnp-tTA promoter is spatially expressed in multiple brain regions, including the cerebral cortex, several basal ganglia regions, hippocampus, tectum, and cerebellum.