16S rDNA sequencing and mass spectrometry-based metabolite profiling of offspring in mice at 24 weeks old

Maternal overnutrition can dramatically increase the susceptibility of offspring to metabolic diseases,whereas maternal exercise may improve glucose metabolism in offspring. C57BL/6 female mice were randomly assigned to four subgroups according to a diet and exercise paradigm before and during pregnancy as follows:NC(fed with normal chow diet and sedentary),NCEx(fed with normal chow diet and running),HF (fed with high-fat diet and sedentary),and HFEx(fed with high-fat diet and running).Integrative 16S rDNA sequencing and mass spectrometry-based metabolite profiling were synchronously performed to characterize the effects of maternal exercise on the gut microbiota composition and metabolite alterations in offspring.