Transcription profiles of WT or MST1/2-KO total, CD8+ and CD8- DCs in vivo

CD8+ DCs play key role in CD8+ T cell priming, however, the underlying signaling mechansim is unclear. We used a data-driven network-based systems biology approach and identified Hippo signaling kinases as key selective modualtors in CD8+ DCs. We focused on Mst1/Stk4 to further investigate the novel function of Hippo signaling in CD8+ DCs. All transcriptional profies were evalated by microarray. 1) We used microarrays of total DCs in previous (GSE98481) and current studies to reverse engineer a DC-specific signaling interactome (DCI). 2) We integrated DCI with profiles of sorted CD8+ with CD8- DCs to identify signaling drivers of CD8+ DCs. 3) We profiled and compared Mst1/2-KO with WT cells from total, CD8+ and CD8- DCs to understand the mechanism of Mst1 in CD8+ DCs. Previous microaarays (GSE98481) and 4 more total DC samples were used for network reconstruction. Four replicates for sorted WT CD8+ and CD8- DCs. Three replicates for Mst1/2-KO total DCs, four replicates for Mst1/2-KO CD8+ and CD8- DCs.