Targetd inactivation of oncogenic drivers in tumor cells derived from adult muscle stem cells

Representative movies of early-passaged tumor propagating cells (TPC) FACS-purified from primary murine tumors. These cells are lineage-traced through activation of a Rosa26-Tomato reporter allele.

"TPC Duxbl" contain copy number amplification of the duxbl gene identified through genomic sequencing.
"TPC Yap1" contain copy number amplification of the yap1 gene identified through genomic sequencing.

These cells were individually subjected to lentiviruses encoding shrnas to inactivate either duxbl or yap1 or to non-coding lentivurs (plko.1 control).

Note that inactivation of duxbl only affected growth of TPC Duxbl but not TPC Yap1. Conversely inactivation of yap1 only affected growth of TPC Yap1l but not TPC Duxbl.