Transcriptome analysis of A549 cells expressing a SUMOylation-deficient TRIM28 mutant in the context of influenza A virus infection

Aim: Identify differentially expressed genes in human lung epithelial cells expressing a SUMOylation-deficient TRIM28 mutant (TRIM28 6KR) compared to TRIM28 wt. Overall design: A CRISPR/Cas9-edited A549 TRIM28 knock-out (KO) single cell clone was reconstituted by lentiviral transduction to express either wild type (wt) TRIM28 or TRIM28 6KR. Cells were infected with influenza A virus (A/WSN/33) at MOI=10 PFU/cell or mock infected and RNA was extracted from three independent replicates at 6 hours post infection (hpi) and analysed by mRNA sequencing.