Detecting fish assemblages with environmental DNA: does protocol matter?

Sequence reference database File containing the sequence reference database for Guianese freshwater fish species. BdR_Guyane.txt Run 1 Compressed file containing: 1) Forward sequence file; 2) Reverse sequence file; 3) File with experiment name and the tags, the forward primer and the reverse primer sequence associated. Compressed file containing: 1) Forward sequence file; 2) Reverse sequence file; 3) File with experiment name and the tags, the forward primer and the reverse primer sequence associated. Run 2 Compressed file containing: 1) Forward sequence file; 2) Reverse sequence file; 3) File with experiment name and the tags, the forward primer and the reverse primer sequence associated. Run 3 Compressed file containing: 1) Forward sequence file; 2) Reverse sequence file; 3) File with experiment name and the tags, the forward primer and the reverse primer sequence associated. Run 4 Compressed file containing: 1) Forward sequence file; 2) Reverse sequence file; 3) File with experiment name and the tags, the forward primer and the reverse primer sequence associated. Run 5 Compressed file containing: 1) Forward sequence file; 2) Reverse sequence file; 3) File with experiment name and the tags, the forward primer and the reverse primer sequence associated. Run 6 Site data A table with 1)Site names, 2)Site codes, 3)Drainage basins, 4)Watercourse types, 5)Strahler order, 6)Latitude, 7)Longitude, 8)Sampling years for P1 samples, 9)Sample PCR codes for P1 samples, 10)Sampling years for P2 samples, 11)Sample PCR codes for P2 samples Sites_data.xls