TGFB signaling hyperactivation-induced tumorigenicity during the derivation of neural progenitors from mouse ESCs

To fully analyze the molecular mechanisms of carcinogenesis of pluripotent stem cells, we enriched the differentation-resistant cells from mouse neural progenitor cells which are derived from Oct4-GFP mouse embryonic stem cell neural differentiation system by sorting GFP positive cells. Transcriptional analyses by RNA sequencing and epigenetic analyses by chromatin immunoprecipitation followed by sequencing for H3K4me3 (Abcam 8580), H3K4me1 (Abcam 8895), H3K27me3 (Millipore 07-449) and H3K27ac (Active Motif 39133) were performed.