Spatiotemporal heterogeneity of inflammatory changes in Alzheimer's disease analyzed spatially resolved transcriptome

We investigated spatiotemporal molecular patterns related to AD pathophsiology using spatially resolved transcriptome of the AD mouse model. The late change of gray matters of AD was commonly related to neuroinflammation, while the early change in the white matter of AD represented neuronal projection and ensheathment of axons before the amyloid plaques accumulation. Disease-associated microglia and astrocyte signatures were spatially differently enriched. Our results provide a key spatiotemporally heterogeneous molecular change particularly related to inflammation in AD. Overall design: Distinctive brain regions were identified and molecular changes in AD model at early (3-month-old) and late (7-month-old) phases were analyzed.