Prometaphase I preparations: spindle configurations.

1One pole is focused while the other end of the spindle is unfocused, see Figure 1B.
2Microtubules are aligned in the same direction but both poles are unfocused, see Figure 1C.
3Only isolated thick bundles of microtubules appear to be projecting from the chromosome mass, see Figure 5B.
4Microtubules are not aligned in a single direction, see Figure 1E.
5Clear microtubules were not associated with the chromosomes despite good antibody penetration.
6One wild-type oocyte was caught in spindle assembly and not included in any column. Lack of bipolarity in the monopolar and barrel-like oocytes was typically due to oocytes lying in poor focal planes for imaging.