Sequence alignment of Lb2Cas12a with other Cas12a orthologs from different organisms.

Multiple sequence alignment by Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) and ESPript 3.0 (http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi). Cas12a proteins sequences from species Lachnospiraceae bacterium MA2020, Lachnospiraceae bacterium ND2006, Moraxella bovoculi, Acidaminococcus sp. BV3L6, Francisella tularensis subsp. novicida U112, respectively. The secondary structure elements are shown above the sequence base on the structure of Lb2Cas12a-crRNA. The domains are shown below the sequence. Arrows indicate catalytic amino acid residues involved function. (ZIP)