Transcriptomic analysis of iPSC and ESC challenged with atmospheric or physiological oxygen

Modulation of metabolism by 5% (physiological) oxygen during reprogramming has been shown to improve reprogramming efficiency, yet it is not known how it may affect the transcriptome and physiology of iPSC. RNA-seq analysis highlighted that iPSC derived under 20% oxygen displayed transcriptomic instability. Consequently, inappropriate modulation of metabolism by atmospheric oxygen during reprogramming significantly impacts the resultant iPSC transcriptional landscape. RNA-Seq analysis of two iPSC lines, and one ESC line, each sub-cultured under 20% and 5% oxygen.