RNA-Seq reveals the role of miR-199a-3p in regulating inflammation of bovine mammary epithelial cells

Purpose: to detect expression profile of differentially expressed mRNAs during bovine mammary epithelial cells transfected with miR-199a-3p mimic or negative control (NC) mimic in vitro. Methods: bovine mammary epithelial cells were transfected with miR-199a-3p mimic or negative control (NC) mimic to assess the expression profiles of mRNAs using RNA-seq. Results: there were 140 DEGs (109 up-regulated and 31 down-regulated) in the miR-199a-3p overexpression group compared with the negative control group. Conclusion: the overexpression of miR-199a-3p resulted in differential expression of 140 genes in bMECs. The functional annotation results of these DEGs suggested that miR-199a-3p may be involved in the inflammatory and immune responses of bMECs by regulating PI3K-Akt signaling pathway, TGF-beta signaling pathway and IL-17 signaling pathway. Bovine mammary epithelial cells were inoculated and cultured in 6-well cell culture plates. When cell confluence reached 60-70%, miR-199a-3p mimic and NC mimic (final concentration 50nM) were transiently transfected into cells. Subsequently, all samples were used to perform the RNA-seq.