SR0726 deletion leads to altered transcriptome of cultured Borrelia burgdorferi

Differential gene expression analysis was performed to assess the affects of the deletion of sRNA SR0726 on the in vitro transcriptome of Borrelia burgdorferi in order to investigate a potential regulatory role for the sRNA Triplicate cultures of wild type and D7 (SR0726 deletion mutant) were grown in BSK-II under standard conditions until mid-log phase, sub-cultured (1:100) to room temperature BSK-II, grown back to mid-log phase, subcultured (1:100) to 35°C, and grown to late-phase. Triplicate cultures for each strain were then pooled and pelleted. RNA was extracted, DNase treated, and was sent to the Novogene Corporation for library preparation, sequencing, and bioinformatic analysis.