Next-generation sequencing of the oscillation zone of arf7 and iaa18/potent reveals new biological processes involved in the root clock function in Arabidopsis

To understand regulation of the in-phase and the antiphase oscillations by IAA18/POTENT and ARF7, we performed next-generation sequencing (NGS). OZ samples were taken at the minimum of DR5::Luciferase expression for the wild type, and simultaneously for arf7-1 and iaa18/potent regardless of DR5::Luciferase expression to avoid bias. A total of nine samples were taken, three independent samples for each of the three cases of study: three samples of Col0 with minimum oscillation, three samples of potent and three samples of arf7.