Effects of inhibitors and changes to the ionic environment on cotransporter phosphorylation.

Phosphorylation levels detected by antibodies R5 and NKCC-P expressed as factors of appropriate controls. Values are given as mean ± s.e.m. (n≥4) or as averages if n = 2.aCells were exposed to ouabain for 35 min.bCells were exposed to Na+-free medium for 5 min.c134 kDa and 274 kDa bands represent monomeric and dimeric fNKCC2A; 155 kDa bands, endogenous NKCC and fNKCC1. As antibodies R5 and NKCC-P detect similar changes in cotransporter phosphorylation data have been pooled.