Supplementary Table S2 for: Therapeutic potential of human adipose-derived stem cell exosomes in stress urinary incontinence – an in vitro and in vivo study

The results of KEGG pathway analysisBackground/Aims:
To
evaluate whether local injection of exosomes derived from human adipose-derived
stem cells (hADSCs) facilitates recovery of stress urinary incontinence (SUI)
in a rat model.

Methods: For the in vitro
study, a Cell Counting Kit-8 (CCK-8) array and proteomic analysis were
performed. For the in vivo study, female rats were divided into four groups:
sham, SUI, adipose-derived stem cell (ADSC), and exosomes (n = 12 each). The SUI model was generated by pudendal nerve
transection and vaginal dilation. Vehicle, hADSCs, or exosomes were injected
into the peripheral urethra. After 2, 4, and 8 weeks, the rats underwent cystometrography
and leak point pressure (LPP) testing, and tissues were harvested for
histochemical analyses.

Results: The CCK-8
experiment demonstrated that ADSC-derived exosomes could enhance the growth of
skeletal muscle and Schwann cell lines in a dose-dependent manner. Proteomic
analysis revealed that ADSC-derived exosomes contained various proteins of
different signaling pathways. Some of these proteins are associated with the
PI3K-Akt, Jak-STAT, and Wnt pathways, which are related to skeletal muscle and
nerve regeneration and proliferation. In vivo experiments illustrated that rats
of the exosome group had higher bladder capacity and LPP, and had more striated
muscle fibers and peripheral nerve fibers in the urethra than rats of the SUI
group. Both urethral function and histology of rats in the exosome group were
slightly better than those in the ADSC group.

Conclusions: Local injection of
hADSC-derived exosomes improved functional and histological recovery after SUI.