High depth sequencing of BrU-labeled nascent RNA.
收藏NIAID Data Ecosystem2026-04-30 收录
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资源简介：
BrU incorporation into nascent RNA followed by immunoprecipitation with a BrU specific antibody. The nascent RNA was subsequently eluted with BrU competition. Overall design: HEK293T cells were incubated with 2mM bromouridine for 15min labeling time and chased with 20mM uridine for different time intervals (0min, 15min, 30min and 60min). Nascent RNA was purified during BrU-IP with anti-BrdU.
创建时间：
2022-10-20
