Screening of differentially expressed genes after IMB5046 treatment. Homo sapiens

To investigate the effects of IMB5046 treatment on cells, we used the gene expression microarray to compare the difference between the IMB5046-treated cells and control cells. A431 cells were treated with 100 nM IMB5046 or 0.1% DMSO (vehicle) for 24 hr. A total of 441 genes were down-regulated and 383 genes were up-regulated by at least 2-fold between IMB5046-treated and control cells.The most obviuos changes in Gene Ontology biolocial process classification were related to cell death and immune response. Overall design: A431 cells were treated with 100 nM IMB5046 or (0.1% DMSO) vehicle for 24 h. Then, total RNA was extracted from cells using TRIzol reagent and analyzed.