Transcriptome analysis of 1-year-old Panax vietnamensis Ha et Grushv.

Purpose: Panax vietnamensis Ha et Grushv., which contains various valuable ginsenosides, is an important herbal medicine of Vietnam. However, it is an endangered species listed in Vietnam Red Data Book due to over-harvseting. Investigation about genomic or trancriptomic resources is one of the necessary activities to conserve P. vietnamensis Materials and Methods:. In this study, we sequenced the transcriptomes of 1-year-old P. vietnamensis from leaves and roots using Illumina NovaSeqTM6000 system. Results: A total of 60,254,062 and 64,588,528 reads was obtained and then assembled into 45,495 and 49,133 unigenes for leaves and roots, respectively. More than 60% unigenes from two organs  were functional annotated using at least one database among Kyoto Encyclopedia of Genes and Genomes, Pfam, Gene Ontology, NCBI non-redundant Protein, and Evolutionary genealogy of genes. Further, the predominant transcripts of each cDNA library were analyzed for different gene expression identification. In addition, 457 unigenes encoding enzymes involved in triterpenoid saponin biosynthesis via the mavelonate (MVA) and the non-MVA (also named as MEP pathways) were discovered. mRNA profiles of 1-year-old P.vietnamensis leaves and roots