Microscopic images of cells and optically-coded microbeads encapsulated in hydrogel droplets

Optical codes can be used to track single live cells in an image data space, while DNA barcodes can be used to identify single cells in a sequencing data space. We have developed dual image and DNA (ID)-coding, which identifies individual single cells in both live image and sequencing data spaces. Samples provided here are relevant to proof-of-concept studies of ID-coding presented in the associated publication. Optically-coded micro-particles were encapsulated in hydrogel droplets with or without single cells. Multiple hydrogel droplets were imaged in individual microscopic views, which were segmented afterwards.