RNA-seq of mouse neutrophils polarized to proinflammatory N1 by lipopolysaccharide and interferon-γ or anti-inflammatory N2 by interleukin-4 versus control. RNA-seq of mouse neutrophils polarized to proinflammatory N1 by lipopolysaccharide and interferon-γ or anti-inflammatory N2 by interleukin-4 versus control

Gene expression profiling for mouse N1 and N2 neutrophils. Neutrphils were isolated form the bone marrow of C57BL/6J mice between 12-16 weeks old. Approximately 8x10^7 neutrophils were used per condition and were split into 3 biological replicates. Freshly isolated neutrophils were cultured for 2h in RPMI medium, in the presence of 100 ng/ml lipopolysaccharide (LPS) and 20 ng/ml interferon gamma (IFNγ) or 20 ng/ml interleukin 4 (IL-4). Total RNA was isolated with TRIzol reagent and Phasemaker tubes. A sample from the LPS+IFNγ polarization did not pass the quality control test and was excluded from the downstream analysis.