mRNA translation efficiency analysis in liver at 0 h and 24 h after 70% partial hepatectomy

In order to detect changes in liver tissue protein translation efficiency during liver regeneration, we performed sucrose density gradient centrifugation to separate translated (associated with polysome fraction), and non-translated (associated with sub-polysome fraction) transcripts in extracts from mouse livers 0 h and 24 h post-surgery. We found that compared to liver tissues before surgery, extracts from tissues 24 h post-surgery had significantly fewer transcripts associated with polysomes, suggesting a reduction in protein synthesis activity. Liver polysome and sub-polysome RNA profiles of mice at 0 h and 24 h after partial hepatectomy were generated by deep sequencing. Three samples from each group were included for sequencing.