Proportions of <i>Lactobacillus</i> recovered from genomic DNA extracted from paired Gram stained slide and vaginal swabs collected from eight healthy women.
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*Preamplification was performed at 98°C for 2 minutes, then followed by 10 cycles at 98°C for 30 seconds and 60°C for 1 minute 30 seconds for 10 cycles using degenerate primers 8F-1492R targetting the bacterial 16S ribosomal gene. **In comparison to average Lactobacillus proportions obtained from vaginal swab (n = 8) without pre amplification. All assays were run in triplicate.
创建时间:
2012-10-11



