DHX15 is involved in SUGP1-mediated RNA missplicing by mutant SF3B1 in cancer
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https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=ffa17f13eee0408980302f8d70f58549
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HEK293T cells were transfected with the plasmid construct expressing FLAG-GST-tagged SUGP1 (aa 543-645), followed by two rounds of affinity purification using FLAG and GST tags. The purified proteins were resolved by SDS-PAGE, followed by staining with QC Colloidal Coomassie Stain (Bio-Rad). The relevant gel region (~85-100 KD) was excised, and the proteins in this gel section were identified by mass spectrometry.
创建时间：
2022-10-07
