Temperature-dependent shifts in multiple tri-trophic defense interactions on black cherry

Many plants engage in tri-trophic interactions whereby plants provide resources such as food or shelter to mutualists in exchange for protection against herbivores and pathogens. Despite their ecological importance, tri-trophic interactions are often context dependent, in which the magnitude and outcome of the interaction can shift under different abiotic and biotic conditions. Disentangling the effects of environmental factors, such as temperature, on tri-trophic interactions is especially important as temperature regimes shift under climate change, but few studies have explored how abiotic shifts affect tri-trophic interactions across multiple trophic groups in the same plant system. Here, we monitored 96 black cherry (Prunus serotina) seedlings for 10 weeks under ambient and increased temperatures to explore the effects of temperature on interactions across multiple trophic levels. Specifically, we explored the effects of temperature on two different tri-trophic interactions between ..., , # Data from: Temperature-dependent shifts in multiple tri-trophic defense interactions on black cherry

Dataset DOI: [10.5061/dryad.18931zd8v](10.5061/dryad.18931zd8v)

## Description of the data and file structure

In June-August 2018, we grew 96 *Prunus serotina* seedlings under either elevated temperature or ambient temperature conditions. Over the course of 10 weeks, we weekly measured the abundance of arthopod predators (spiders and ants), the amount of herbivory plants experienced, and plant height. At the conclusion of the experiment, we collected a subset of 3 leaves per plant and assessed mite abundance, the abundance of fungal hyphae, the presence and size of leaf domatia, and extrafloral nectary abundance on each leaf. We combine these datasets to assess the effect of warming on the abundances and interactions between these groups.

### Files and variables

#### File: Dawson-Glass_warming_cherries_analyses_070125.R

**Description:** Script with data preparation and statistic...,