Hypoxia-induced translational profiles of murine fetal liver derived macrophages (FLDM)

We report changes in total and translated poly(A) RNA in mouse fetal liver derived macrophages after exposure to hypoxia. We employed translating ribosome affinity purification (TRAP) to isolate polysomal RNA from mouse fetal liver derived macrophages after exposure to hypoxia. Overall design: Total and translated poly(A) RNA samples were isolated from day E14.5 FLDM isolated from 3 conditional HuR-knockout (Csf1r-iCre.HuRfl/fl) and 2 control mice (Csf1r-iCre.HuR+/+) expressing ribosomal protein L10a fused to EGFP. FLDM were differentiated in vitro and exposed to normoxia or hypoxia (1% O2) conditions for 18 h.