Jennifer Bestman, Holly Cline (2011) CIL:36951, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #1 (day 1) of a time lapse series contained with in this image group. It is a pair of developing neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The images were acquired daily over 3 days and show dendritic arbor development.

The cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

本图组包含了一系列时间序列影像中的第1张图像（第1天）。该图像呈现了一对来自无色斑马鱼（Xenopus laevis）中枢神经系统视束发育中的神经元。图像拍摄于47期发育阶段。该影像组中的图像每日采集，持续3天，用以展示树突分支的生长发育过程。在首次采集图像前约24小时，通过原位电穿孔法将编码eYFP的质粒DNA转染至细胞内。活体蝌蚪在麻醉状态下，置于玻璃载片下方，并通过特制双光子显微镜直接穿过其透明皮肤进行拍摄。