Effect of YAP5SA expression on the transcriptome of proliferating and senescent cells

We have generated IMR90-hTERT cells carrying a Doxycyclin-inducible (Tet-On) vector expressing an N-terminally Flag tagged YAP5SA. To identify differentially expressed genes by YAP5SA expression during cellular senescence, we performed transcriptional profiling by RNA sequencing (RNA-seq) with or without YAP5SA expression (using doxycyclin), before and after senescence induction using a stress-induced senescence model (bleomycin-induced senescence). IMR90-hTERT cells carrying a Dox-inducible (Tet-On) vector expressing an N-terminally Flag tagged YAP5SA were grown with or without Dox and treated with bleomycin (10 ug/ml for 24 h) to induce senescence. Total RNA was isolated using the acid phenol extraction method. One microgram of high-quality RNA samples (RIN > 7.0) was used to construct RNA-seq libraries using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina). RNA-seq was performed on an Illumina NovaSeq 6000 sequencer.