Comparison of human pluripotent stem cell-derived cardiomyocyte subtypes

Different subtypes of cardiomyocytes were induced from human induced pluripotent stem cells. Human induced pluripotent cells were basically differentiated using cardic differentiation protocol with a GSK3 inhibitor and a Wnt inhibitor (GiWi). Cells were purified using no glucose medium supplemented with lactate. Standard GiWi mainly produced NKX2-5-positive/TBX5-positive cardiomyocytes and modified GiWi mainly produced NKX2-5-positive/TBX5-negative cardiomyocytes.